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KMID : 0545119970070020101
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 2 p.101 ~ p.106
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Strain Improvement for Enhanced Production of Streptokinase and Streptodornase in Streptococcus sp.
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Hyun, Hyung Hwan
Lee, Yoon Bum/Song, Kyung Hwa/Jeon, Ji Young/Lee, Hyune Hwan
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Abstract
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Strain improvement for the enhanced production of streptokinase and streptodornase in Streptococcus sp. ATCC 12449 was performed. Strain UB111, a hyperproductive mutant which was isolated by use of nitrosoguanidine and selection of colonies with large clear zones on DNase test agar plates supplemented with 1% glucose and 0.5% ammonium chloride, produced about 3 fold more streptokinase and streptodornase than the wild type when tested in shake flask fermentations. The enhanced production of both streptokinase and streptodornase was achieved by cultivating the mutant in a pH-controlled fermentor containing fermentation medium enriched with yeast extract (2.1%). Under these conditions, the mutant produced 7300 units/㎖ of streptokinase and 800 units/㎖ of streptodornase.
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